Part:BBa_K2381000

pMagFast

pMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52R/M55R. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52D/M55G. They can heterodimerize under blue light irritation (470 nm, 3 mW/cm²). Their light sensing molecule is FAD which is normal in nearly all organisms. This part is pMag-Fast, whose dissociation rate is more rapid than the origin pMag. It should be used together with nMag BBa_K2381001.It's function is proved by parts BBa_K2381017
If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker, and remember to add FAD.

Reference

Kawano, F., Suzuki, H., Furuya, A., & Sato, M. (2015). Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat Commun, 6, 6256. doi:10.1038/ncomms7256

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 87
  
• 1000
  COMPATIBLE WITH RFC[1000]